While yeasts can vary in size, they typically measure 3 to 8 µm in diameter. Yeasts are single celled eukaryotic fungi organisms that reproduce asexually by budding or division (Figure 1). This application note describes the use of the Agilent BioTek Lionheart FX automated microscope and the ONIX2 microfluidic platform to monitor yeast cell growth through the analysis of kinetic brightfield images of yeast cells under a variety of conditions in real time. Likewise, the development of new strains often requires monitoring the growth of strains under various conditions. The ability to obtain the desired product reliably and repeatedly requires careful monitoring of not only the input materials, but the growth of the yeast strain during the process. Optimization of these processes often involves the development of strains that are capable of growth under a wide variety of environmental conditions. Strains of the yeast Saccharomyces cerevisiae serve critical roles in the production of many different products including food staples, medicines, and biofuels. Using the Agilent BioTek Lionheart FX automated microscope and the ONIX2 system to image and analyze yeast cell growthĪuthor: Paul Held Ph.D, Agilent Technologies, Inc.
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